Custom Protein Production
Cloning
Expression & Purification
Activity Testing
Bulk Production
With Optinum Insight llC custom protein development services, we use optimized conditions to generate recombinant proteins with high purity and high yields. Projects are composed of five milestones where data is provided after each milestone completion. Each project is customizable through working directly with our team of highly trained scientists.
PROJECT MILESTONES
1. Cloning
Unless provided, Optinum Insight llC can supply the image clone or synthetic DNA. Mutations will be introduced if needed. Confirmation of successful cloning will be completed through gene sequencing. For baculovirus generation and Sf9 insect cell expression projects, the plasmid will be transformed into competent cells, purified, and screened for positive clones using PCR.
Protein Tags: His, GST, Myc, FLAG, Avi, MBP, Fc-fusion
2. Expression & Purification
The plasmids will be transfected through using lipofectamine, electroporation, or lentiviral transduction with the desired expression system.
Expression Systems
- Mammalian (HEK293 or CHO-K1)
- E. coli
- Baculovirus/Sf9 insect cells
Next, the cells will be harvested and the proteins purified. If necessary, Optinum Insight llC can perform buffer exchange and concentrate the samples.
Customizable Purification Options
- Additional rounds of column purification
- Endotoxin testing
- Inclusion body purification
- Biotinylation (Avi-Tag or Side Chain) and pull down QC testing
- Protein refolding
- Phosphorylation and dephosphorylation
- Protein labeling services
Biotinylation: Biotin label your protein specifically using Avi-TagTM technology or through specific amino acid functional-groups. We can create custom labels for streptavidin binding, purification, surface plasmon resonance, and conjugation.
Fluorescence Labeling: Let Optinum Insight llC help you choose from our wide selection of dyes. Label your proteins either through biotin-streptavidin binding or through direct conjugation. This is useful for fluorescence imaging, fluorescence-based biochemical screens (e.g. trFRET), or FACS analysis.
Antibody Labeling: The Optinum Insight llC team are experts at direct attachment of dyes to primary and secondary antibodies for epitope optimization, imaging, and ELISA replacements.
Enzyme Conjugation: Rapidly develop immunoassays with Horseradish Peroxidase conjugated to your protein or antibody.
3. Activity Testing
Choose to test your custom protein using Optinum Insight llC collection of >200 biochemical and cell-based assays, or we can develop a new custom activity assay. Additionally Optinum Insight llC can provide thermal shift assays if desired.
4. Bulk Protein Production
Optinum Insight llC has the capabilities to scale up protein production and produce the product in bulk. Our team of scientists will re-express and re-purify the protein while making any necessary adjustments to the formulation to meet any necessary specifications.
5. Special Requirements
Optinum Insight llC offers many additional options for protein expression.
Customizable Options
- Mutagenesis studies
- Protein conjugation with probes and dyes
- Protein binding and protein interaction studies
Contact us to learn more about how we can create a custom protein for you